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Abstract

A rapid and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative estimation of Picroside-II, Phyllanthin and Hypophyllanthin simultaneously in marketed formulation (Valliliv capsule) containing Picrorhiza kurroa and Phyllanthus niruri. The sample (Valliliv capsule) were chromatographed on silica gel 60F254- TLC plates, using solvent system (Hexane: Ethyl acetate, 6:3 v/v) and scanned at 278nm. The linearity range for Picroside was 200-1600ng/spot with average recovery of 99.90%, Phyllanthin was 200-1600ng/spot with average recovery 100.41% and Hypophyllanthin 200-1600ng/spot with average recovery of 99.99% in formulation. The limit of detection and limit of quantification for Picroside-II, Phyllanthin and Hypophyllanthin were found to be 40ng/spot- 120ng/spot, 90-270ng/spot and 100ng/spot-300ng/spot. The developed method was successfully applied for the assay of market formulation containing Picrorhiza kurroa and Phyllanthus niruri.

Keywords

Fingerprinting Hepatoprotective HPTLC Phyllanthin TLC Hypophyllanthin Picroside Method development Validation

Article Details

How to Cite
Safeena Sheikh, Subuddha Yadav, Suhail Asghar, & Showkat Ahmad. (2013). Development of validated HPTLC method for quantitation of phyllanthin, hy-pophyllanthin and picroside-II in marketed herbal formulation (Valiliv cap-sules). International Journal of Research in Pharmaceutical Sciences, 4(1), 50-57. Retrieved from https://pharmascope.org/ijrps/article/view/1115